CovidV1, Main, Exploration, bibRecord, 001567

Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader

Identifieur interne : 001567 ( Main/Exploration ); précédent : 001566; suivant : 001568

Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader

Auteurs : Beate Schwer ; Paolo Visca ; Jan C. Vos ; Hendrik G. Stunnenberg

Source :

Cell [ 0092-8674 ] ; 1987.

RBID : ISTEX:E9CD905B90E0DB155F32B782018B4C3BB7217F57

English descriptors

Teeft :
- Acad, Binding buffer, Cdna, Dhfr, Discontinuous transcripts, Eukaryotic, Experimental procedures, First strand cdna, Genome, Genomic, Hanggi, Homopolymeric stretches, Hybrid, Late transcripts, Leader sequence, Mapping experiments, Mrna, Natl, Nucleotide, Primer, Primer elongation, Primer extension, Proc, Promoter, Promoter sequences, Readthrough, Readthrough transcripts, Recombinant, Recombinant virus, Rnaase, Rnaase treatment, Sequencing, Size markers, Taaat, Taaat motif, Transcript, Transcriptase, Transcription, Transcription initiation, Translocated, Translocated promoter, Vaccinia, Vaccinia virions, Vaccinia virus.

Abstract

Abstract: We have demonstrated by primer elongation and cap analysis that mature vaccinia virus late transcripts are discontinuously synthesized. We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the “start site” determined by S1 mapping using vaccinia genomic DNA as a probe. Sequencing of the RNA and of the first strand cDNA reveal that a homopolymeric poly(A) sequence is linked to the 5′ terminus of the RNA transcripts. S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position −1, confirms the existence of a poly(A) leader of approximately 35 A-residues.

Url:

https://api.istex.fr/ark:/67375/6H6-D81LTP1V-V/fulltext.pdf

DOI: 10.1016/0092-8674(87)90212-1

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000683
to stream Istex, to step Curation: 000663
to stream Istex, to step Checkpoint: 000705
to stream Main, to step Merge: 001590
to stream Main, to step Curation: 001567

Le document en format XML

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<term>Eukaryotic</term>
<term>Experimental procedures</term>
<term>First strand cdna</term>
<term>Genome</term>
<term>Genomic</term>
<term>Hanggi</term>
<term>Homopolymeric stretches</term>
<term>Hybrid</term>
<term>Late transcripts</term>
<term>Leader sequence</term>
<term>Mapping experiments</term>
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<term>Primer elongation</term>
<term>Primer extension</term>
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<term>Promoter sequences</term>
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<term>Vaccinia</term>
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<term>Vaccinia virus</term>
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<front><div type="abstract" xml:lang="en">Abstract: We have demonstrated by primer elongation and cap analysis that mature vaccinia virus late transcripts are discontinuously synthesized. We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the “start site” determined by S1 mapping using vaccinia genomic DNA as a probe. Sequencing of the RNA and of the first strand cDNA reveal that a homopolymeric poly(A) sequence is linked to the 5′ terminus of the RNA transcripts. S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position −1, confirms the existence of a poly(A) leader of approximately 35 A-residues.</div>
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<name sortKey="Vos, Jan C" sort="Vos, Jan C" uniqKey="Vos J" first="Jan C." last="Vos">Jan C. Vos</name>
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Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader

Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri